Journal: JCI Insight

Article Title: Matrix metalloproteinases are hallmark early biomarkers and therapeutic targets in FSHD

doi: 10.1172/jci.insight.195104

Figure Lengend Snippet: ( A ) UMAP plot of merged single-cell data from uninduced WT (control) and dox-induced iDUX4 mice (10 days on 625 mg/kg dox chow), with clusters annotated by cell types. ( B ) UMAP comparison of cell clusters between control and DUX4-induced muscles. ( C ) Proportional representation of major cell populations in control and DUX4-induced muscles. ( D and E ) UMAP plots showing the spatial distribution and cellular origin of MMP expression in control ( D ) and DUX4-induced ( E ) muscles. ( F ) Bubble plots highlighting distinct MMP expression patterns across cell types. ( G ) Normalized fold-change in MMP expression within selected cell populations. ( H ) Violin plots showing expression levels of Mmp2 , Mmp14 , and Mmp19 across annotated cell clusters. Data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 by Student’s 2-tailed t test. ECs, endothelial cells; MSCs, mesenchymal stem cells; SCs, satellite cells; UMAP, uniform manifold approximation and projection.

Article Snippet: For immunofluorescence, tissue sections or sorted FAPs were fixed in 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 for 30 minutes, and incubated overnight at 4°C with primary antibody against collagen VI (1:200, Proteintech), MMP2 (1:500, Proteintech), MMP14 (1:500, Proteintech), PDGFRα (1:200, BioLegend), F4/80 (1:200, BioLegend), and Ki-67 (1:400, BioLegend), followed by secondary antibody conjugate to Alexa Fluor 488, 555, or 647 (1:500, Thermo Fisher Scientific) for 2 hours at room temperature.

Techniques: Control, Comparison, Muscles, Expressing

Journal: Frontiers in Oncology

Article Title: Overexpression of MMP14 is associated with poor prognosis and immune cell infiltration in colon cancer

doi: 10.3389/fonc.2025.1564375

Figure Lengend Snippet: Single-cell transcriptome analysis of the relationship between MMP14 and colorectal cancer. (A) CD45 negative non-immune cell annotated tSNE; (B) Expression of MMP14 in different cell subsets of tSNE; (C) The expression of MMP14 among different cell subsets; (D) Fibroblasts subgroup subdivision; (E) The expression of MMP14 in different subpopulations after Fibroblasts subgroups; (F) Fib6 ratio among different samples; (G) Fib6 and other Fib difference analysis volcano map; (H) GO enrichment analysis of differential genes; (I) KEGG enrichment analysis of differential genes.

Article Snippet: Additionally, to further examine MMP14 protein expression levels in CRC, we examined the Human Protein Atlas (HPA) database, focusing on MMP14 protein expression in various malignancies. ( www.proteinatlas.org/ ).

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: Overexpression of MMP14 is associated with poor prognosis and immune cell infiltration in colon cancer

doi: 10.3389/fonc.2025.1564375

Figure Lengend Snippet: Single-cell transcriptome analysis of the relationship between MMP14 and colorectal cancer. (A) CD45-positive immune cell annotated tSNE; (B) Histogram of the proportional accumulation of immune cells between different samples; (C) A 50-point bitmap of the significant difference of immune cells between the two groups. Statistical significance is denoted as **P < 0.01. ns means no significant difference, p greater than 0.05.

Article Snippet: Additionally, to further examine MMP14 protein expression levels in CRC, we examined the Human Protein Atlas (HPA) database, focusing on MMP14 protein expression in various malignancies. ( www.proteinatlas.org/ ).

Techniques:

Journal: Frontiers in Oncology

Article Title: Overexpression of MMP14 is associated with poor prognosis and immune cell infiltration in colon cancer

doi: 10.3389/fonc.2025.1564375

Figure Lengend Snippet: Development of prognostic gene markers based on MMP14-associated co-expression genes and MMP14. (A) Univariate Cox regression analysis to assess prognostic significance; (B, C) LASSO assays for feature selection; (D) Multivariate Cox regression analysis to construct prognostic model; (E, F) Evaluation of the impact of the prognostic model on OS and PFS; (G) ROC curves for model validation. (H-J) Heat map illustrating expression patterns of six genes, the distribution of risk score, and the survival status of CRC patients.

Article Snippet: Additionally, to further examine MMP14 protein expression levels in CRC, we examined the Human Protein Atlas (HPA) database, focusing on MMP14 protein expression in various malignancies. ( www.proteinatlas.org/ ).

Techniques: Expressing, Selection, Construct, Biomarker Discovery

Journal: Frontiers in Oncology

Article Title: Overexpression of MMP14 is associated with poor prognosis and immune cell infiltration in colon cancer

doi: 10.3389/fonc.2025.1564375

Figure Lengend Snippet: Experimental detection of MMP14 expression in clinical samples. (A) qRT-PCR for MMP14 expression detection; (B) Western blot for detection of MMP14 expression; (C) Immunofluorescence for the detection of MMP14 expression, with DAPI staining for cellular localization; (D) H&E staining of three pairs of cancerous and adjacent tissues. Statistical significance is denoted as *P < 0.05, **P < 0.01.

Article Snippet: Additionally, to further examine MMP14 protein expression levels in CRC, we examined the Human Protein Atlas (HPA) database, focusing on MMP14 protein expression in various malignancies. ( www.proteinatlas.org/ ).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Immunofluorescence, Staining

Journal: Frontiers in Oncology

Article Title: Overexpression of MMP14 is associated with poor prognosis and immune cell infiltration in colon cancer

doi: 10.3389/fonc.2025.1564375

Figure Lengend Snippet: Construction of MMP14-silenced CRC cell system. (A) qRT-PCR detection of IL-1 β mRNA expression; (B) Knockdown of MMP14 detected by qRT-PCR; (C) Flow cytometry was used to detect cell apoptosis following MMP14 silencing. Statistical significance is denoted as **P < 0.01, and ****P < 0.0001.

Article Snippet: Additionally, to further examine MMP14 protein expression levels in CRC, we examined the Human Protein Atlas (HPA) database, focusing on MMP14 protein expression in various malignancies. ( www.proteinatlas.org/ ).

Techniques: Quantitative RT-PCR, Expressing, Knockdown, Flow Cytometry

Journal: Frontiers in Oncology

Article Title: Overexpression of MMP14 is associated with poor prognosis and immune cell infiltration in colon cancer

doi: 10.3389/fonc.2025.1564375

Figure Lengend Snippet: Single-cell transcriptome analysis of the relationship between MMP14 and colorectal cancer. (A) CD45 negative non-immune cell annotated tSNE; (B) Expression of MMP14 in different cell subsets of tSNE; (C) The expression of MMP14 among different cell subsets; (D) Fibroblasts subgroup subdivision; (E) The expression of MMP14 in different subpopulations after Fibroblasts subgroups; (F) Fib6 ratio among different samples; (G) Fib6 and other Fib difference analysis volcano map; (H) GO enrichment analysis of differential genes; (I) KEGG enrichment analysis of differential genes.

Article Snippet: This was further corroborated by Human Protein Atlas (HPA) data, which confirmed notably higher MMP14 protein abundance in CRC compared to normal tissues.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: Overexpression of MMP14 is associated with poor prognosis and immune cell infiltration in colon cancer

doi: 10.3389/fonc.2025.1564375

Figure Lengend Snippet: Single-cell transcriptome analysis of the relationship between MMP14 and colorectal cancer. (A) CD45-positive immune cell annotated tSNE; (B) Histogram of the proportional accumulation of immune cells between different samples; (C) A 50-point bitmap of the significant difference of immune cells between the two groups. Statistical significance is denoted as **P < 0.01. ns means no significant difference, p greater than 0.05.

Article Snippet: This was further corroborated by Human Protein Atlas (HPA) data, which confirmed notably higher MMP14 protein abundance in CRC compared to normal tissues.

Techniques:

Journal: Frontiers in Oncology

Article Title: Overexpression of MMP14 is associated with poor prognosis and immune cell infiltration in colon cancer

doi: 10.3389/fonc.2025.1564375

Figure Lengend Snippet: Development of prognostic gene markers based on MMP14-associated co-expression genes and MMP14. (A) Univariate Cox regression analysis to assess prognostic significance; (B, C) LASSO assays for feature selection; (D) Multivariate Cox regression analysis to construct prognostic model; (E, F) Evaluation of the impact of the prognostic model on OS and PFS; (G) ROC curves for model validation. (H-J) Heat map illustrating expression patterns of six genes, the distribution of risk score, and the survival status of CRC patients.

Article Snippet: This was further corroborated by Human Protein Atlas (HPA) data, which confirmed notably higher MMP14 protein abundance in CRC compared to normal tissues.

Techniques: Expressing, Selection, Construct, Biomarker Discovery

Journal: Frontiers in Oncology

Article Title: Overexpression of MMP14 is associated with poor prognosis and immune cell infiltration in colon cancer

doi: 10.3389/fonc.2025.1564375

Figure Lengend Snippet: Experimental detection of MMP14 expression in clinical samples. (A) qRT-PCR for MMP14 expression detection; (B) Western blot for detection of MMP14 expression; (C) Immunofluorescence for the detection of MMP14 expression, with DAPI staining for cellular localization; (D) H&E staining of three pairs of cancerous and adjacent tissues. Statistical significance is denoted as *P < 0.05, **P < 0.01.

Article Snippet: This was further corroborated by Human Protein Atlas (HPA) data, which confirmed notably higher MMP14 protein abundance in CRC compared to normal tissues.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Immunofluorescence, Staining

Journal: Frontiers in Oncology

Article Title: Overexpression of MMP14 is associated with poor prognosis and immune cell infiltration in colon cancer

doi: 10.3389/fonc.2025.1564375

Figure Lengend Snippet: Construction of MMP14-silenced CRC cell system. (A) qRT-PCR detection of IL-1 β mRNA expression; (B) Knockdown of MMP14 detected by qRT-PCR; (C) Flow cytometry was used to detect cell apoptosis following MMP14 silencing. Statistical significance is denoted as **P < 0.01, and ****P < 0.0001.

Article Snippet: This was further corroborated by Human Protein Atlas (HPA) data, which confirmed notably higher MMP14 protein abundance in CRC compared to normal tissues.

Techniques: Quantitative RT-PCR, Expressing, Knockdown, Flow Cytometry